| 摘要 |
<P>PROBLEM TO BE SOLVED: To solve a problem in that soyasapogenol B is bio-synthesized by the ring closure of 2,3-oxidosqualene produced from β-amyrin which is a bio-synthetic precursor of the soyasapogenol B, through a mevalonic acid pathway and further through two stage hydroxylation reactions, however a gene of 22 position hydroxylase associating with the reaction is not yet elucidated. <P>SOLUTION: It is found out that the 22 position of an oleanane type triterpene is hydroxylated by specifying the gene of the hydroxylase of 22 position of the oleanane type triterpene and co-expressing by combining with a specific gene. Further, it is found out that by co-expressing the gene of the 22 position hydroxylase with the gene of 24 position hydroxylase, the soyasapogenol B can be produced in a large amount and in a good efficiency. <P>COPYRIGHT: (C)2011,JPO&INPIT |
