METHOD OF STIMULATING CHRONIC SPINAL CORD LESION AND TRAINING TO TREAT IT IN SMALL LABORATORY ANIMALS

摘要

FIELD: medicine. ^ SUBSTANCE: rats' spinal cord is cut completely at the inferior thoracic level Th7. IT is followed with electrical stimulation with electrodes placed on a dorsal surface of the spinal cord on segments L2 and S1, as well as with introduction of quipazine and 8-OHDRAT. Hind limbs of an animal rest against a treadmill. Quipazine is administered intraperitoneally 0.3 mg/kg, 8-OHDRAT is introduced 5 min later subcutaneously 0.1-0.3 mg/kg, and electrical stimulation is started 10 min later. Spinal cord segments L2 and S1 are stimulated simultaneously by rectangular pulses of duration 0.2 ms, frequency 40-50 Hz, intensity 1-4 V. An integrated effect is started from the 7th day following a cutting procedure and carried on daily within a therapeutic course of 7 days. ^ EFFECT: method allows to induce interrelated step motions. ^ 2 dwg, 1 ex