ANALYTICAL ELEMENT AND ITS USE IN A WHOLE BLOOD HEMOGLOBIN ASSAY

摘要

ANALYTICAL ELEMENT AND ITS USE IN A WHOLE BLOOD HEMOGLOBIN ASSAY Disclosed herein is an assay useful for the quantitative determination of hemoglobin in whole blood. This assay utilizes a multizone element comprising a registration zone comprising particulate barium sulfate distributed in a "blush" polymer, and a spreading zone having a void volume and an average pore size (e.g. greater than 5 microns) effective to accommodate whole blood. The element also contains an interactive composition capable of converting substantially all forms of hemoglobin into a detectable end product. One particularly useful interactive composition comprises a hemoglobin oxidizing agent (e.g. ferricyanide) and thiocyanate. Another useful interactive composition comprises an ionic surfactant, e.g. sodium octyl sulfate or sodium dodecyl sulfate.