发明名称 |
siRNA DETECTION METHOD |
摘要 |
PolydG is added to the terminal overhangs of an siRNA. Next, a primer containing a polydC sequence added with a tag sequence is annealed and cDNA is synthesized by a reverse transcription reaction. Quantitative PCR is performed between a primer carrying the same sequence as the tag sequence, and a primer containing the same sequence as the siRNA sequence to be detected. The amount of siRNA of interest can be determined from a calibration curve produced using known amounts of short-chain dsDNA.
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申请公布号 |
US2011033858(A1) |
申请公布日期 |
2011.02.10 |
申请号 |
US20090866087 |
申请日期 |
2009.01.29 |
申请人 |
GENECARE RESEARCH INSTITUTE CO., LTD. |
发明人 |
FUTAMI KAZUNOBU;FURUICHI YASUHIRO |
分类号 |
C12Q1/68;C07H21/00;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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