IMPROVED DETECTION OF BACTERIAL (MOLLICUTES) CONTAMINATION

摘要

The present invention provides an improved PCR-based amplification of a target sequence by suppressing non-specific amplification products. The improvement concerns the use of a primer pair optimized to amplify a nucleic acid of a contaminant in the background of genomic DNA of a first organism. When DNA from a second organism suspected for comprising the contaminant is subjected to the same PCR-based amplification reaction, detection sensitivity and specificity of the contaminant is enhanced when an amount of genomic DNA of the first organism is present in the amplification reaction.