IMMUNOASSAY-BASED ANTIGEN DETECTING KIT AND METHOD

摘要

PURPOSE: A kit for detecting an antigen and a detection method using the same are provided to simultaneously detect several antigens by multiple-OLISA(oligonucleotide-linked immunosorbent assay). CONSTITUTION: A kit for detecting an antigen comprises: a support; a capture antibody(c-Ab) which is fixed on the support; a detection antibody-DNA conjugate(d-Ab-DNA); a fluorescence-RNA-quencher conjugate; and RNase H. The kit is able to detect two or more kinds of antigens. A method for detecting the antigen comprises: a step of contacting a sample to the capture antibody which is able to selectively conjugate to the antigen to be detected to conjugate the capture antibody and antigen in the sample; a step of conjugating the antigen with d-Ab-DNA; a step of contacting the antibody with fluorescence-RNA quencher conjugate to hybridize DNA and RNA; a step of treating with RNase H to cut RNA and release fluorescence from RNA; and a step of measuring fluorescence intensity.