XYLANASE GENE SEQUENCES FROM THE GENOMIC DNA OF UNPURIFIED RUMEN MICROORGANISMS

摘要

A xylanase gene, denoted xynR8, encoding a novel xylanase (XynR8) obtained from the unisolated rumen microorganisms is provided. The DNA sequence of the xynR8 gene, xylanase, is also provided, the enzyme is thermo-tolerable, and highly specific for xylans with high activity. Transformation of microbial hosts with the xynR8 gene is described. A method for degrading the xylan-containing structure comprises hydrolyzing the .beta.-1,4- glycosidic bonds of xylans by contacting xylanase is provided, and a composition employing the above-mentioned hydrolyzing method is further addressed.