摘要 |
Provided is an immunoassay for detecting a test substance with high sensitivity. The immunoassay is a method of detecting or measuring a test substance contained in a sample liquid, and includes: reacting, with the test substance, a first specifically binding substance which is immobilized on a support and is capable of binding specifically to the test substance and a second specifically binding substance which is labeled with a labeling substance and is capable of binding specifically to the test substance; separating, from the support, the second specifically binding substance which is unreactive with the test substance; eluting the labeling substance retained on the support; depositing the eluted labeling substance on a test element; and measuring electrochemically a catalyst reaction amount of the labeling substance deposited on the test element.
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