发明名称 |
PROBES AND METHODS FOR DETECTION OF PATHOGENS AND ANTIBIOTIC RESISTANCE |
摘要 |
Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.
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申请公布号 |
US2011027782(A1) |
申请公布日期 |
2011.02.03 |
申请号 |
US20100788101 |
申请日期 |
2010.05.26 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;U.S. GOVERNMENT REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS |
发明人 |
HAAKE DAVID A.;CHURCHILL BERNARD M.;LIAO JOSEPH C.;SUCHARD MARC A.;LI YANG;MASTALI MITRA |
分类号 |
C12Q1/68;C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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