发明名称 |
MUTANT METHYLGLYOXAL SYNTHASE (MGS) FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION |
摘要 |
The present invention concerns a method for the production of a biochemical selected among lactic acid, acetol and 1,2-propanediol, comprising culturing a microorganism modified for an improved production of the biochemical selected among lactic acid, acetol and 1,2-propanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a methylglyoxal synthase (MGS) enzyme which activity is not inhibited by orthophosphate. The present invention concerns a mutant methylglyoxal synthase (MGS) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein - the mutant enzyme has retained more than 50% of the methylglyoxal synthase activity of the parent enzyme and - the methylglyoxal synthase activity of the mutant MGS is not inhibited by orthophosphate as compared to the parent enzyme. |
申请公布号 |
CA2767962(A1) |
申请公布日期 |
2011.02.03 |
申请号 |
CA20102767962 |
申请日期 |
2010.07.30 |
申请人 |
METABOLIC EXPLORER |
发明人 |
VOELKER, FRANCOIS;DUMON-SEIGNOVERT, LAURENCE;SOUCAILLE, PHILIPPE |
分类号 |
C12N9/00;C12N9/88;C12N15/00;C12P7/02;C12P7/56 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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