| 摘要 |
A method for generating a population of single stranded DNA molecules representative of a DNA sample, including digesting the DNA sample with a Type I restriction endonuclease; subjecting the DNA fragments to melt conditions to produce a melt-generated mixture of forward and reverse single stranded DNA fragments; contacting the mixture of forward and reverse single strand DNA fragments with a solid support having immobilized thereon the complement of either the forward or reverse strand, wherein the solid phase hybridizations are favored over self-annealing of the melt-generated single stranded DNA molecules; and releasing the captured forward or reverse single strand DNA fragments from the complement immobilized to the solid support to generate a population of single stranded DNA molecules representative of the DNA sample.
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