发明名称 |
MUTANT GLYCEROL DEHYDROGENASE (GLYDH) FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION |
摘要 |
The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a micro organism modified for an improved production of 1,2- propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced. The present invention also relates to a mutant glycerol dehydrogenase(Gly DH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein - the mutant enzyme has retained more than 50% of the glycerol dehydrogenase activity of the parent enzyme and - the glycerol dehydrogenase activity of the mutant GlyDH is less inhibited by NAD+ and/or by its substrate as compared to the parent enzyme and/or by its product as compared to the parent enzyme. |
申请公布号 |
WO2011012702(A1) |
申请公布日期 |
2011.02.03 |
申请号 |
WO2010EP61129 |
申请日期 |
2010.07.30 |
申请人 |
METABOLIC EXPLORER;VOELKER, FRANCOIS;DUMON-SEIGNOVERT, LAURENCE;MEYNIAL-SALLES, ISABELLE;SOUCAILLE, PHILIPPE |
发明人 |
VOELKER, FRANCOIS;DUMON-SEIGNOVERT, LAURENCE;MEYNIAL-SALLES, ISABELLE;SOUCAILLE, PHILIPPE |
分类号 |
C12N15/53;C07C29/60;C12N1/21;C12N9/04;C12P7/18;C12Q1/32 |
主分类号 |
C12N15/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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