FLOW-THROUGH IMMUNO-ELECTRODE FOR FAST AMPEROMETRIC IMMUNOASSAYS

摘要

An electrode (15, 19) and measuring cell (17, 50) for fast flow immunoassay of a wide range of analytes. The high surface area electrode (15, 19) comprises an electroconductive material with immobilized immuno-species deposited on glass, paper or plastic filter (18, 62). The solution containing the target analyte and/or the enzyme labeled immunoconjugate flows through the electrode (15, 19). The assay procedure is based on a competitive or sandwich scheme. The immuno-interaction between the immobilized immuno-species target analyte and the enzyme labeled immunoconjugate results in binding of the enzyme label to the electrode surface. The assay of the enzyme label is performed by flow injection of the corresponding enzyme substrate, and amperometric measurement of the product of the enzymatic reaction.