| 摘要 |
Measuring the half-life of fluorescence emission in the frequency domain is made difficult by the presence of background signals. A method and apparatus are proposed for detecting said lifetime even when the fluorescence is much lower than the background, without the need for a calibration sample, based on successive exploration of states in the system. The feature of these states is that the background signal is constant, whereas the signal of interest changes. The signal of interest may be recovered by cancelling the common signal. This method may be used to eliminate the effect of light dispersed by excitation, autofluorescence and electromagnetic interference. |
