发明名称 Method Of Transfecting Cells With Nucleic Acids Using Acidified Polyethylenimine
摘要 An object of the present invention is to achieve a method capable of increasing transfection efficiency of polyethyleneimine (PEI) and reducing toxicity thereof, to provide a PEI with high transfection efficiency, and to provide a method of introducing nucleic acids into cells comprising using the PEI. There are provided a method of introducing nucleic acids into cells comprising mixing acidified PEI with nucleic acids under acidic conditions and applying the resulting mixture to cells, a reagent for introducing nucleic acids into cells comprising an acidified PEI solution, and a reagent kit including the solution.
申请公布号 US2011020927(A1) 申请公布日期 2011.01.27
申请号 US20100752188 申请日期 2010.04.01
申请人 NATIONAL UNIVERSITY CORPORATION CHIBA UNIVERSITY 发明人 YAMAGUCHI NAOTO;FUKUMOTO YASUNORI
分类号 C12N5/10 主分类号 C12N5/10
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