| 摘要 |
FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention relates to biotechnology, specifically to obtaining proteolytic fractions from procariotic cells, and can be used in analysis of molecular-genetic mechanisms of formation of procaryote cell structures and the role of protein components in their organisation, which is needed to obtain additional information in design and construction of computer models of organisation of gene and epigene control networks. Escherichia coli cells are preserved in the presence of buffered 80-90% glycerin and then treated with 3% triton X-100 in order to remove the cell membrane. Further, the obtained cytoplasmic proteins are extracted with increasing concentrations of salts: 0.14 M, 0.35 M; 2 M NaCl, 6 M guanidine hydrochloride with 0.1% -mercaptoethanol. Affinity chromatography on sepharose 4B with immobilised trypsin inhibitor is carried out and proteolytic activity is evaluated. ^ EFFECT: invention enables to obtain a fraction from procaryote cells. ^ 4 dwg, 3 tbl |
