| 摘要 |
FIELD: medicine. ^ SUBSTANCE: method includes oophorectomy in animals after slaughter, transportation of oophorons, extraction of oocyte-cumulus complexes by aspiration of follicles content, irrigation of the complexes and their cultivation in a medium not covered with oil, in the presence of serum gonadotropin of pregnant mares, human chorionic gonadotropin and 5% estrous serum of bovine cattle. At that, follicles of diametre from 2 to 8 mm are used to obtain oocytes. After extraction of the oocyte-cumulus complexes are immediately placed in a washing medium containing 0.5 mM of isobutylmethylxanthine to synchronise reinitiation of meiosis. Before the cultivation the selection of oocytes based on their morphological evaluation is carried out. All the procedures of isolation, irrigation and selection are carried out in a medium containing 0.5 mM of isobutylmethylxanthine. Oocyte-cumulus complexes were cultured in a medium modified by insertion of 50 ng/ml of bovine prolactin hormone. ^ EFFECT: increase of proportion of mature oocytes in vitro, and their ability for further development. ^ 1 ex, 1 tbl |
