发明名称 |
METHODS FOR TRANSFERRING GENE INTO CHROMOSOME |
摘要 |
<p>Method for transferring a foreign gene into a host chromosome by contrived procedures to integrate the foreign gene into a vector by utilizing a lysogenic phage, for the purpose of deleting unnecessary genes derived from the vector or the like, except for the foreign gene. One of the inventive method comprises: a first step for preparing a vector with a lysogenic phage integration site (attP site) arranged between a first partial sequence of the objective foreign gene to be transferred but lacking one terminal region thereof and a second partial sequence of the gene but lacking the other terminal region and having an overlapping region with a portion of the first partial sequence; a second step for integrating the vector obtained in the first step into the host chromosome; and a third step for screening from among the recombinants obtained in the second step a recombinant from which unnecessary genes originating in the vector have been deleted owing to the homologous recombination mechanism functioning in the overlapping region between the first and second partial sequences. <IMAGE></p> |
申请公布号 |
EP0974666(A1) |
申请公布日期 |
2000.01.26 |
申请号 |
EP19970928454 |
申请日期 |
1997.06.25 |
申请人 |
KABUSHIKI KAISHA YAKULT HONSHA |
发明人 |
KADOTA, MARIKO;KIWAKI, MAYUMI;SAWAKI, SAEKO;SHIRASAWA, YUKIO;SONE, HARUE;SAKO, TOMOYUKI |
分类号 |
C12N1/21;C12N15/74;C12N15/90;(IPC1-7):C12N15/74 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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