发明名称 |
Nucleic acid amplification reaction station for disposable test devices |
摘要 |
An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
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申请公布号 |
US2011020921(A1) |
申请公布日期 |
2011.01.27 |
申请号 |
US20100924351 |
申请日期 |
2010.09.24 |
申请人 |
BIOMERIEUX, INC. |
发明人 |
KLUTTZ BRYAN W.;MCKINLEY GEOFF A.;GENNARI FABIO;GUY MICHEL;COTTER CHRISTOPHER;CATANZARITI LUIGI;GRAZIANO LOUIS;COLIN BRUNO;PARIS CECILE;DACHAUD JACQUES |
分类号 |
C12M1/34;C12N15/09;B01J19/00;B01J19/24;B01L3/00;B01L7/00;C12M;C12M1/00;C12M1/18;C12M3/00;C12Q1/68;G01N21/00;G01N33/50;G01N35/02;G01N37/00 |
主分类号 |
C12M1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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