A PROCESS FOR PREPARING STEM CELL BASED FORMULATIONS
摘要
The present invention discloses a process for preparing a formulation comprising stem cells the process comprises the steps of: subjecting a bone marrow aspirate to centrifugation to separate supernatant and cellular fraction comprising stem cells; isolating the stem cells; subjecting the supernatant to a plurality of centrifugation steps wherein following each centrifugation step the supernatant is freezed and thawed rapidly to remove cellular matter and obtain an acellular supernatant; reconstituting the stem cells with acellular supernatant. The formulation thus prepared comprises stem cells suspended in an acellular bone marrow supernatant which is devoid of any other type of contaminating cells such as T cells or cellular component, however it would have the extrinsic factors present in the bone marrow. The formulation is in a ready to use form for clinical application for use in stem cell based therapies.