METHOD FOR THE CONSTRUCTION OF RANDOMIZED GENE SEQUENCE LIBRARIES IN CELLS

摘要

An in vivo method for the construction of randomized gene libraries and/or domain replacement in gene libraries by homologous recombination using a Kluyveromyces lactis killer toxin, in particular the γ-subunit of the K. lactis killer toxin, as negative selection marker is described. The use of the γ-subunit of K. lactis as negative selectable marker increases the percentage of randomized clones.