发明名称 |
Methylated CpG Island Amplification (MCA) |
摘要 |
The present invention provides a method for identifying a methylated CpG containing nucleic acid by contacting a nucleic acid with a methylation sensitive restriction endonuclease that cleaves unmethylated PcG sites and contacting the sample with an isoschizomer of the methylation sensitive restriction endonuclease, which cleaves both methylated and unmethylated CpG sites. The method also includes amplification of the CpG-containing nucleic acid using CpG-specific oligonucleotide primers. A method is also provided for detecting an age associated disorder by identification of a methylated CpG containing nucleic acid. A method is further provided for evaluating the responses of a cell to an agent. A kit is useful for detection of a CpG containing nucleic acid is also provided. Nucleic acid sequences encoding novel methylated clones.
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申请公布号 |
US2011020807(A1) |
申请公布日期 |
2011.01.27 |
申请号 |
US20100763917 |
申请日期 |
2010.04.20 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE |
发明人 |
ISSA JEAN-PIERRE;BAYLIN STEPHEN;TOYOTA MINORU |
分类号 |
C12N15/09;C12Q1/68;C07H21/00;C07K14/00;C12N1/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/63;C12Q1/48 |
主分类号 |
C12N15/09 |
代理机构 |
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