| 摘要 |
FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention relates to genetic engineering. Disclosed are expression DNA structures which include a T-shaped DNA structure with a fragment (19-23 bases)of the target gene sequence subject to transcription with formation of siRNA, which provide specific inhibition of said gene in a cell via RNA interference. Described is a method of obtaining the disclosed DNA structure, which does not involve PCR steps and is a simple three-step procedure which is performed in a single reaction vessel. ^ EFFECT: invention can be used for practical purposes for inhibiting production of target proteins in cells. ^ 14 cl, 7 dwg, 1 ex |
