ISOLATION OF UNKNOWN REARRANGED T-CELL RECEPTORS FROM SINGLE CELLS
摘要
<p>Disclosed herein are methods and materials for isolating and identifying T cell receptors from single cells. In some embodiments, genomic DNA from a single T cell is isolated using whole genome amplification (WGA). A series of PCR reactions is carried out to enrich the genomic template for sequences encoding the TCR alpha and beta chains, and then to isolate the sequences encoding the TCR alpha and beta chains.</p>
申请公布号
WO2011008502(A2)
申请公布日期
2011.01.20
申请号
WO2010US40245
申请日期
2010.06.28
申请人
CALIFORNIA INSTITUTE OF TECHNOLOGY;BALAZS, ALEJANDRO, BENJAMIN;TSAI, JONATHAN, MICHAEL;BALTIMORE, DAVID
发明人
BALAZS, ALEJANDRO, BENJAMIN;TSAI, JONATHAN, MICHAEL;BALTIMORE, DAVID