发明名称 |
ISOLATION OF UNKNOWN REARRANGED T-CELL RECEPTORS FROM SINGLE CELLS |
摘要 |
Disclosed herein are methods and materials for isolating and identifying T cell receptors from single cells. In some embodiments, genomic DNA from a single T cell is isolated using whole genome amplification (WGA). A series of PCR reactions is carried out to enrich the genomic template for sequences encoding the TCR alpha and beta chains, and then to isolate the sequences encoding the TCR alpha and beta chains.
|
申请公布号 |
US2011014659(A1) |
申请公布日期 |
2011.01.20 |
申请号 |
US20100824744 |
申请日期 |
2010.06.28 |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY |
发明人 |
BALAZS ALEJANDRO BENJAMIN;TSAI JONATHAN MICHAEL;BALTIMORE DAVID |
分类号 |
C12P19/34;C07H21/04 |
主分类号 |
C12P19/34 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|