| 摘要 |
FIELD: medicine. ^ SUBSTANCE: there is offered a method for producing adult stem cells with multipotent properties that involves recovering thereof from human brown adipose tissue and cultivating in a nutrient medium containing N-acetyl-L-cysteine, as well as a method for long-term maintaining these cells in the undifferentiated condition due to formation of spheres in a CORM-2-containing medium. The adult stem cells produced by the offered method are characterised by high proliferation rate, positive CD73, CD90, CD29, CD44 and CD 105 immune response, negative CD33, CD34, CD45, CD4, CD31, CD62p, CD14 and HLA-DR immune response and ability to differentiate in cells of mesodermal origin. Particularly, disclosed is a possibility of producing nervous cells, cartilaginous cells, osteogenous cells, adipose cells and insulin-producing pancreatic cells of said adult stem cells under the invention. ^ EFFECT: method improvement. ^ 12 cl, 15 dwg, 2 tbl, 10 ex |
