1-HEXADECYL-5-(1-PYRENEBUTYL)-N-(L-ORNITILE)-L-GLUTAMATE BISCHLORIDE

摘要

FIELD: chemistry. ^ SUBSTANCE: lipodipeptide is 1-hexadecyl-5-(1-pyrenebutyl)-N-(L-ornitile)-L-glutamatebischloride, which can deliver nucleic acids into cells and contains in its structure a stable fluorescent mar in form of pyrene. The obtained compound is 10 times less toxic to Hela and HEP cells compared to Lipofectamine. The synthesised compound has more efficient transfection on neoplastic (HeLa, HEP, COS-7) and nonneoplastic cell lines (CHO, rat leucocyte) than special commercial transfection agents for HEP and CHO cells. ^ EFFECT: owing to presence of a stable fluorescent mark in the structure of the synthesised compound, the field of using such substances widens considerably and it becomes possible to study localisation of labelled liposomes and duration of the transfection process. ^ 1 ex