摘要 |
The present invention relates to a method for producing double haploid plants, comprising the steps of allowing pollen with one functional sperm cell to fertilize an embryo sac cell which is not the central cell; allowing the central cell to proliferate into endosperm; and regenerating a double haploid plant from the endosperm.The pollen with one functional sperm cell is for example mutant pollen, which is obtainable by chemical mutation, transformation with a nucleic acid, or irradiation. |