发明名称 |
PROCESSES FOR REFOLDING OF INSULIN |
摘要 |
<p>The invention provides processes for obtaining a precursor for insulin, analogs or derivatives thereof having correctly bonded cystine bridges. The process involves solubilizing a precursor of insulin, insulin analog or derivatives in an aqueous solution or a buffer containing cysteine or cysteine hydrochloride and one or more of chaotropic auxiliary. The solubilized precursors are refolded by adding diluent to the solubilized mixture (reverse dilution). Further, the solubilized precursors, wherein the concentration of precursor in reaction mixture is more than 0.65 g/litre can also be refolded by diluting the reaction mixture with a diluent optionally comprising about 5-40% v/v of one or more of alcoholic or aprotic solvents.</p> |
申请公布号 |
EP2274318(A2) |
申请公布日期 |
2011.01.19 |
申请号 |
EP20090738542 |
申请日期 |
2009.04.30 |
申请人 |
WOCKHARDT RESEARCH CENTRE |
发明人 |
EDUPUGANTI B, RAJU;JAGIRDAR, HASEEB;KUMAR, MANISH;PARTHIPAN, JAYARAMAN;YADAV, VIVEK;SAHIB, MAHARAJ K |
分类号 |
C07K1/113 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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