发明名称 |
Enhanced cell-free synthesis of active proteins containing disulfide bonds |
摘要 |
Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.
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申请公布号 |
US7871794(B2) |
申请公布日期 |
2011.01.18 |
申请号 |
US20080016763 |
申请日期 |
2008.01.18 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
KNAPP KURTIS G.;SWARTS JAMES ROBERT |
分类号 |
C07K1/00 |
主分类号 |
C07K1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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