发明名称 |
Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins |
摘要 |
The present invention provides methods and compositions for generating novel nucleic acid molecules through RNA trans-splicing that target a highly expressed pre-mRNA and contain the coding sequence of a protein or polypeptide of interest. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with the target precursor messenger RNA molecule (target pre-mRNA) that is abundantly expressed, and mediate a trans-splicing reaction resulting in the generation of novel chimeric RNA molecule (chimeric RNA) capable of encoding a protein or polypeptide of interest. The invention provides for the in vivo production of chimeric RNA molecules that encode and result in the production of a protein or polypeptide of interest.
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申请公布号 |
US7871795(B2) |
申请公布日期 |
2011.01.18 |
申请号 |
US20050245907 |
申请日期 |
2005.10.07 |
申请人 |
VIRXSYS CORPORATION |
发明人 |
MCGARRITY GERARD J.;GARCIA-BLANCO MARIANO A. |
分类号 |
C12P21/06;C07H21/04;C12N9/00 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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