THE METHOD OF EXTRACTING MEDICAL ACTIVE MATERIAL FROM CIRSIUM JAPONICUM VAR. USSURIENSE KITAMURA AND THE FUNCTIONAL FOODS USING IT

摘要

PURPOSE: A method for extracting female hormone from Cirsium japonicum is provided to obtain a pharmacologically active material of high value added. CONSTITUTION: A method for extracting silymarin from Cirsium japonicum comprises: a step of collecting and washing Cirsium japonicum and drying in an oven at 70°C for three days; a step of pulverizing dried Cirsium japonicum to obtain dried Cirsium japonicum sample; a step of maintaining the dried Cirsium japonicum sample at constant temperature and moisture degree; a step of refluxing the dried Cirsium japonicum sample using 70-90% of methanol at 50-70°C for 100-150 minutes two or five times; a step of cooling the methanol solution by room temperature and concentrating; and a step of adding 130-200 ml of ethyl acetate.