Device, array, and methods for disease detection and analysis

摘要

A device and array coupled to capture molecules are provided. Specifically, the device and array can be used for detecting the presence and concentration of biomarkers in a sample from a subject. The device and array can also allow the use of a method for scoring a sample for, e.g., the purpose of diagnosing a disease. The method can also be advantageous to applications where there is a need to accurately determine the disease stage of a subject for the purpose of making therapeutic decisions.

主权项

1. A multiplex immunoassay detection device for simultaneous measurement of a plurality of distinct breast cancer markers in a patient serum sample, comprising:
a glass solid support consisting of an array of antibody Clone 191924 for HER2, antibody Clone 36006.211 for MMP-2, antibody Clone M8071022 for CA-15-3 and antibody Clone 190312 for osteopontin; a glass cover plate, wherein the glass cover plate forms an upper surface positioned above the solid support; a vertical support comprising adhesive silicone, wherein the vertical support forms a connection between the solid support and the cover plate, the connection forming at least one channel surrounding the array of antibody Clone 191924 for HER2, antibody Clone 36006.211 for MMP-2, antibody Clone M8071022 for CA-15-3 and antibody Clone 190312 for osteopontin, and wherein the at least one channel comprises a first end and a second end and wherein the first end of the at least one channel comprises an opening; an absorbent material comprising a nitrocellulose membrane connected to the second end; and a set of biotinylated detection antibodies for detection of breast cancer markers bound to antibody Clone 191924 for HER2, antibody Clone 36006.211 for MMP-2, antibody Clone M8071022 for CA-15-3 and antibody Clone 190312 for osteopontin, the detection antibodies consisting of antibody clone AF-1129 for HER2, antibody clone AF-902 for MMP-2, antibody Clone M8071021 for CA 15-3 and antibody Clone AF-1433 for osteopontin.