METHOD FOR DIFFERENTIATION OF POLYNUCLEOTIDE STRANDS
摘要
Objective of the present invention is to provide a method for keeping of directional information in double-stranded DNA. We suggest to convert polynucleotide into a hybrid double-stranded DNA. One particular strand of this hybrid double-stranded DNA should be synthesised using at least one modified nucleotide. Thus, this particular strand would contain modified nucleotides along the whole length. Density of directional markers would not depend on the length of polynucleotides. Any internal fragments of the hybrid double-stranded DNA would have directional information. When it is necessary the modified strand may be easily degraded or separated from the other strand. It was found that such hybrid double-stranded DNA may be easily generated in a number of molecular biology tasks and may be used for molecular cloning, library preparation and strand separation.
申请公布号
WO2011003630(A1)
申请公布日期
2011.01.13
申请号
WO2010EP04399
申请日期
2010.07.06
申请人
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E. V.;SOLDATOV, ALEKSEY;BORODINA, TATIANA;LEHRACH, HANS