发明名称 |
REFOLDING PROTEINS USING A CHEMICALLY CONTROLLED REDOX STATE |
摘要 |
<p>A method of refolding proteins expressed in non-mammalian cells present in concentrations of 2.0 g/L or higher is disclosed. The method comprises identifying the thiol pair ratio and the redox buffer strength to achieve conditions under which efficient folding at concentrations of 2.0 g/L or higher is achieved and can be employed over a range of volumes, including commercial scale.</p> |
申请公布号 |
CA2765881(A1) |
申请公布日期 |
2011.01.13 |
申请号 |
CA20102765881 |
申请日期 |
2010.06.21 |
申请人 |
AMGEN INC. |
发明人 |
SHULTZ, JOSEPH EDWARD;HART, ROGER;KEENER, RONALD NIXON, III |
分类号 |
C07K1/113 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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