| 发明名称 |
Compostions and methods of nucleic acid-targeting nucleic acids |
| 摘要 |
This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof. |
| 申请公布号 |
US9410198(B2) |
申请公布日期 |
2016.08.09 |
| 申请号 |
US201514751055 |
申请日期 |
2015.06.25 |
| 申请人 |
Caribou Biosciences, Inc. |
发明人 |
May Andrew Paul;Haurwitz Rachel E.;Doudna Jennifer A.;Berger James M.;Carter Matthew Merrill;Donohoue Paul |
| 分类号 |
C12N9/22;C12N15/52;C12Q1/68;C12N15/85;C12N15/10;A61K38/46;A61K47/48;C12N15/90 |
主分类号 |
C12N9/22 |
| 代理机构 |
|
代理人 |
Fabian Gary R.;McClung Barbara G. |
| 主权项 |
1. A method for identifying off-target binding sites relative to a target DNA target sequence, comprising:
contacting a mixture of DNA fragments comprising the target DNA sequence with a complex comprising an enzymatically inactive Cas9 protein and a nucleic acid-targeting nucleic acid, wherein (i) the complex comprises an affinity tag, and (ii) the complex binds to the target DNA sequence; purifying the DNA fragments bound by the complex using the affinity tag; and identifying the off-target binding sites based on the DNA fragments bound by the complex that do not comprise the target DNA binding sequence. |
| 地址 |
Berkeley CA US |
