VERFAHREN ZUR HERSTELLUNG VON PRÄPARATIONEN REKOMBINANTER AAV-VIRIONEN, DIE WEITGEHEND FREI VON LEEREN CAPSIDEN SIND

摘要

Methods for separating AAV empty capsids from mixtures of AAV vector particles and AAV empty capsids are described. The methods use column chromatography techniques and provide for commercially viable levels of recombinant AAV virions.