| 摘要 |
FIELD: biotechnology.SUBSTANCE: present invention relates to biotechnology. Disclosed is a method of preparing a purified conjugate in a solution, involving bringing into contact a maytansinoid molecule containing a thiol group, with a heterobifunctional linker reagent for covalent bonding of linker to maytansinoid molecule, then conjugation of antibody with maytansinoid molecule associated with linkers, by reacting obtained crude mixture with antibody in a solution having pH from approximately 4 to approximately 9, and applying to produced mixture tangential flow filtration, dialysis, gel filtration, adsorption chromatography, selective deposition or combination thereof to produce purified conjugate.EFFECT: disclosed method requires no purification of intermediate compounds, provides high output of conjugates of high purity and homogeneity without inter-chain crosslinking and inactivated residues of linker and can find further application in production of medicinal preparations based on corresponding conjugates.9 cl, 25 dwg, 1 tbl, 8 ex |
