| 摘要 |
<p>The present invention described and claimed herein relate to the design and construction of nucleic acid probes to Staphylococcus aureus which are capable of detecting the organism in test samples of, e.g., sputum, urine, blood and tissue sections, food, soil and water. The present invention design the overall probe composed of specific functional subsequences comprising the target recognition site, two universal primer sequence, two locking sequences and a 5' flanking sequence. This probe design allow the probe amplification, by means of a universal primer pair, only in the presence of the target sequence. Multiple probes can be used simultaneously which can be distinguished by means of length differences (PCR) or by means of the binding of another labelled probe (real-time quantitative PCR). This method is characteristic of fast, easily operating, high sensitivity, high specificity, and the interpretation of the result accurate and reliable. It's suitable for large scale screen in both clinical or agriculture applications.</p> |
