Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes

摘要

Provided are devices and methods for effecting processing of samples, including essentially isothermal amplification of nucleic acids, at multiple reaction locations in a single device. In some embodiments, the disclosed devices and methods provide for effecting parallel sample processing in several hundred locations on a single device.

主权项

1. A method, comprising:
dispersing a first sample that comprises at least one molecule of interest among a plurality of first areas in both a first substrate and a second substrate, at least one of the plurality of first areas containing one and only one molecule of interest, wherein dispersing said first sample comprises exerting the sample through a first conduit in fluidic communication with the plurality of first areas via a first continuous fluidic path within said first and second substrates: dispersing a reactant material into a plurality of second areas in both said first substrate and said second substrate; and effecting pairwise placement of at least some of the plurality of first areas into direct fluidic communication with at least some of the plurality of second areas so as to form closed reaction chambers and contact reactant material with the first sample, wherein the pairwise placement comprises relative motion between the first and second substrates which isolates the plurality of first areas disposed in the first substrate from the plurality of first areas disposed in the second substrate.