Production of saturated fatty alcohols from engineered microorganisms

摘要

Recombinant bacterial microorganisms are provided which comprise heterologous fatty acyl reductases (“FAR”) polypeptides wherein said microorganisms have been engineered to produce increased amounts of saturated fatty alcohols and methods of making saturated fatty alcohols using the recombinant bacterial microorganisms.

主权项

1. An engineered bacterial microorganism comprising at least one polynucleotide encoding at least one heterologous fatty acyl-ACP reductase enzyme (FAR), wherein said FAR comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO:28 and has fatty alcohol forming acyl-CoA reductase activity, and a first recombinant polynucleotide encoding a FabZ enzyme, wherein said FabZ enzyme comprises the amino acid sequence set forth in SEQ ID NO:8, wherein the engineered bacterial microorganism produces a fatty alcohol composition comprising C10 to C18 fatty alcohols.