Methods or analyzing polar metabolites of the engergy metabolism

摘要

The present invention is concerned with the analysis of polar metabolites and provides methods for analyzing polar metabolites comprising extracting a biological sample with a extraction buffer comprising a phase separator and a volatile ammonium salt under conditions which allow for immediate disruption of cells comprised by the biological sample, separating the polar metabolites comprised by the extract by chromatography, and analyzing the separated polar metabolites. Moreover, a method for quenching a biological sample comprising cellular material is contemplated.