摘要 |
<p>The present invention relates to a method for HLA typing of HLA-DRB1 the unambiguous determination of short DNA sequence elements (2-6 bases) at a given position simultaneously on both parental alleles at a selected number of positions in HLA-DRB1 gene, comprised of the steps for each position of a) hybridising a combination of oligonucleotides (primers) complementary to all known sequence variants to a DNA strand upstream of a given position; b) carrying out a primer extension reaction with at least one of the four dNTP substrates substituted by a terminating analog; c) analysing the products by mass spectrometry, with the resulting masses allowing unambiguous identification of the used primers and the added bases. The method may be used to generate subgroups A-P using 10 sequence elements, or to completely resolve all alleles using further additional sequence elements.</p> |