| 摘要 |
<p>The invention provides systems, methods and kits for the separation and/or purification of double-stranded and single-stranded nucleic acids. The method includes first mixing a sample containing the double-stranded nucleic acid and the single-stranded nucleic acid with a pH-neutral, buffered solution consisting essentially of a chaotropic salt and a pH buffer to generate a mixture; then applying the mixture to a first mineral support for the double-stranded nucleic acid to bind; and collecting the flow-through which contains unbound single-stranded nucleic acid. The method further includes adjusting the pH of the flow-through to an acidic pH, and applying the acidified flow-through to a second mineral support for the single-stranded nucleic acid to bind. Alternatively the flow-through can be mixed with a lower aliphatic alcohol prior to loading of the second column. The double-stranded and the single-stranded nucleic acids bound can be eluted from the mineral supports respectively.</p> |
