| 摘要 |
Embodiments are directed to a methods and systems for nucleic acid detection using enzymatic reactions on a microarray. In one embodiment, a probe comprising a probe nucleotide sequence and a substantially homogenous sequence extender portion is provided on the surface of a microarray. The probe nucleotide sequence is hybridized to the complementary target nucleotide sequence. A solution containing enzymes and detection elements is applied to the hybridized probe structure. The enzyme determines the composition of the nucleotide structure of the extender and creates a complementary homogenous sequence extender structure between the target nucleotide sequence and the microarray surface structure. The detection elements in the solution are bound to the extender structure, thus allowing detection using an appropriate detector system.
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