METHOD FOR REPLICATING, AMPLIFYING AND SEQUENCING A TEMPLATE DNA

摘要

The invention relates to the field of biotechnology, specifically to a method for carrying out the replication, amplification or sequencing of a desoxyribonucleic acid with a f29 DNA polymerase. According to said method, said polymerase is incubated in a reaction mixture comprising polyoxyethylenated sorbitane monolaurate (Tween 20) in a concentration of between 0.003 and 0.01%, together with an ammonium salt that can be 30-60 mM ammonium sulfate, 60-120 mM ammonium chloride or ammonium acetate, among other components. The invention also relates to a kit for carrying out said method.