IMPROVED PRODUCTION OF MALTOTETRAOSE SYRUP USING A PSEUDOMONAS SACCHAROPHILA MALTOTETRAOHYDROLASE VARIANT AND A DEBRANCHING ENZYME

摘要

The combination of a Pseudomonas saccharophila G4-forming amylase (PS4) variant and a pullulanase advantageously can catalyze at a high temperature saccharification to produce an increased amount of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant supplement with a pullulanase is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.