Method of pretreating sample for measurement of glycated amine and method of measuring glycated amine

摘要

The present invention provides a method of measuring an amount of a glycated protein as an analyte in a sample, wherein the method comprises a pre-treatment step comprising causing a fructosyl amino acid oxidase (FAOD) to degrade and remove a glycated amino acid that can be present in the sample and which does not derive from the glycated protein; a degradation step with a protease to degrade the glycated protein to give a glycated peptide and/or a glycated amino acid; and a measurement step which comprises causing the FAOD to act on the glycated peptide and/or glycated amino acid to cause a redox reaction, which is measured to determine the amount of glycated protein.