| 摘要 |
An object of the present invention is to provide a peptide derivative for determining &bgr;-glucan or endotoxin which allows high sensitivity measurement, and a method for determining &bgr;-glucan and/or endotoxin using the same. The present invention relates to (1) a peptide derivative represented by the following general formula [1]: X-A1-Gly-Arg-A2-E-D  [1], (2) a reagent for determining &bgr;-glucan and/or endotoxin comprising the above-described peptide derivative, (3) a method for determining &bgr;-glucan and/or endotoxin, characterized in that a sample containing &bgr;-glucan and/or endotoxin, an amebocyte lysate of a horseshoe crab and the above-described peptide derivative are reacted each other, then the resulting released compound represented by the following general formula [2]: H-A2-E-D  [2] is separated from unreacted substance and quantified, and the determination is made based on this value, and (4) a reagent kit for determining &bgr;-glucan and/or endotoxin, comprising an amebocyte lysate of a horseshoe crab and the above-described peptide derivative as constituents thereof.
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