COMPOSITIONS AND METHODS FOR DETECTING BACKGROUND MALE DNA DURING FETAL SEX DETERMINATION

摘要

This invention provides compositions and methods for distinguishing fetal from non-fetal male DNA in a human maternal whole blood sample. The invention provides isolated oligonucleotides and compositions thereof complementary to a centromeric alphoid repeat sequence of the human Y chromosome, which can be used to hybridize, amplify and/or detect non-fetal male DNA present in a blood sample in an amplification assay. The invention also provides methods for detecting or determining the presence or absence of non-fetal DNA in a maternal blood sample.